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2015.03.31생명공학부2643
- 일시 : 2015.04.03
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◈
주 제 : Investigations
on the putative role of photosystem II Manganese Stabilizing Protein in plant
development employing biochemical and biophysical approaches.
◈ 연 사 : Mayank Anand Gururani
◈ 소 속 : 영남대학교
◈ 일 시 : 2015년 4월 3일(금) 오후 3시30분
◈ 장 소 : 영남대학교 생명공학관 333호
< Abstract >
The
33 kDa Manganese-stabilizing protein (MSP) represents a key component of the
oxygen-evolving complex (OEC) and overall photosynthetic machinery. MSP is
primarily involved in the water splitting step where electrons are released
from PSII and transported to PSI via electron transport chain. Individual transgenic
potato lines with sense and anti-sense expression of MSP were generated to
investigate the putative role of MSP in overall plant growth and abiotic stress
tolerance. MSP antisense plants were found to exhibit higher tuber yield with
increased total soluble carbohydrates. Photosynthetic efficiencies examined
through the chlorophyll-a transient kinetics (JIP-test) revealed that
MSP-antisense plants were photosynthetically more active than the MSP-sense and
control (UT) plants. The oxygen measurements indicated that the relative oxygen
evolution was directly proportional to the MSP expression. Chlorophyll-a
fluorescence analyses indicated a possible lack of intact OECs in MSP antisense
plants, which allows access to internal non-water electron donors and
consequently increases the PSII activity in those plants. Furthermore, the MSP-As
plants challenged to different abiotic stress treatments exhibited significant
level of tolerance. Higher specific enzyme activities of major ROS scavenging
enzymes and increased accumulation of low molecular weight metabolites in
MSP-As indicated the improved level of stress tolerance. Primary photochemical
efficiencies estimated through OJIP kinetics analyses confirmed that MSP-As
plants were in better optimal physiology under stress compared to the control
plants. Taken together, these findings strongly indicate the role of MSP in
plant growth, tuberization and abiotic stress tolerance, however further
investigations are warranted to elucidate the precise molecular basis behind increased
stress tolerance in stressed MSP-As plants.
영남대학교 생명공학연구소장
▲문의처 : TEL : 810-3598 (생명공학연구소http://biotech.yu.ac.kr)