[생명공학연구소] 생명공학연구소 제5차 세미나 안내
2015.05.11생명공학연구소2895
- 일시 : 2015.05.18
생명공학연구소에서는 다음과 같이 세미나를 개최하고자 하오니 교수님 및 학생 여러분들의 많은 참석 바랍니다.
◈ 주 제 : Unraveling the complex biology of infection and pathogenesis by the rice blast pathogen Magnaporthe oryzae
◈ 연 사 : Ralph A. Dean
◈ 소 속 : 영남대학교
◈ 일 시 : 2015년 5월 18일(월) 오후 4시
◈ 장 소 : 영남대학교 자연자원대 제 3실험동 214호
< Abstract >
Rice blast disease is caused by the filamentous Ascomycetous fungus Magnaporthe oryzae and results in significant annual rice yield losses worldwide. Infection by this and many other fungal plant pathogens requires the development of a specialized infection cell called an appressorium. The molecular processes regulating appressorium formation are incompletely understood. We analyzed genome-wide gene expression changes during spore germination and appressorium formation on a hydrophobic surface compared to induction by cAMP. During spore germination, 2,154 (approximately 21%) genes showed differential expression, with the majority being up-regulated. During appressorium formation, 357 genes were differentially expressed in response to both stimuli. These genes, which we refer to as appressorium consensus genes, were functionally grouped into Gene Ontology categories. Overall, we found a significant decrease in expression of genes involved in protein synthesis. Conversely, expression of genes associated with protein and amino acid degradation, lipid metabolism, secondary metabolism and cellular transportation exhibited a dramatic increase. We functionally characterized several differentially regulated genes, including a subtilisin protease (SPM1) and a NAD specific glutamate dehydrogenase (Mgd1), by targeted gene disruption. These studies revealed hitherto unknown findings that protein degradation and amino acid metabolism are essential for appressorium formation and subsequent infection. We present the first comprehensive genome-wide transcript profile study and functional analysis of infection structure formation by a fungal plant pathogen. Our data provide novel insight into the underlying molecular mechanisms that will directly benefit efforts to identify fungal pathogenicity factors and aid the development of new disease management strategies.
영남대학교 생명공학연구소장
▲문의처 : TEL : 810-2988, 3030 (생명공학연구소 http://biotech.yu.ac.kr)