미생물생명공학전공 세미나 일정 알림
2015.11.17생명공학연구소3612
- 일시 : 2015.12.11
미생물생명공학전공 세미나 일정 알림
일자: 12월 11일 오전 11시
장소: 생명공학부 YB room
연사: 최용진박사 (미국 UC버클리대학교)
세미나제목: Deficiency of microRNA miR-34a expands cell fate potential in pluripotent stem cells
Abstract
Mouse embryonic stem cells (ESCs) and induced pluripotent stem cells possess pluripotent cell fate potential, efficiently contributing to all embryonic cell types, but rarely to extra-embryonic lineages 1-3. Here, we identify a microRNA, miR-34a, whose deficiency in mouse pluripotent stem cells expands their developmental potential. In a variety of in vitro and in vivo functional assays, miR-34a-deficient pluripotent stem cells exhibit totipotent-like cell fate potential both at the population level and at the single cell level, giving rise to both embryonic and extra-embryonic cell lineages. The expression profiles of miR-34a-/- pluripotent stem cells are characterized by a strong yet specific induction of MuERV-L (MERVL) family endogenous retroviruse, a unique molecular hallmark of totipotent 2-cell stage blastomeres and totipotent like ESCs 4,5. We demonstrate that miR-34a represses MERVL expression through transcriptional regulation, at least in part by directly repressing the transcription factor GATA-binding protein 2 (Gata2). Consistent with the strong correlation between MERVL activation and totipotent-like fate potential. the miR-34a/Gata2 pathway that represses MERVL expression also restricts the acquisition of totipotency in pluripotent stem cell culture. Taken together, our findings provide novel insights into the molecular basis for totipotent-like cell fate potential, and highlight the functional importance of miRNAs in regulating cell fate plasticity in pluripotent stem cell culture.
장소: 생명공학부 YB room
연사: 최용진박사 (미국 UC버클리대학교)
세미나제목: Deficiency of microRNA miR-34a expands cell fate potential in pluripotent stem cells
Abstract
Mouse embryonic stem cells (ESCs) and induced pluripotent stem cells possess pluripotent cell fate potential, efficiently contributing to all embryonic cell types, but rarely to extra-embryonic lineages 1-3. Here, we identify a microRNA, miR-34a, whose deficiency in mouse pluripotent stem cells expands their developmental potential. In a variety of in vitro and in vivo functional assays, miR-34a-deficient pluripotent stem cells exhibit totipotent-like cell fate potential both at the population level and at the single cell level, giving rise to both embryonic and extra-embryonic cell lineages. The expression profiles of miR-34a-/- pluripotent stem cells are characterized by a strong yet specific induction of MuERV-L (MERVL) family endogenous retroviruse, a unique molecular hallmark of totipotent 2-cell stage blastomeres and totipotent like ESCs 4,5. We demonstrate that miR-34a represses MERVL expression through transcriptional regulation, at least in part by directly repressing the transcription factor GATA-binding protein 2 (Gata2). Consistent with the strong correlation between MERVL activation and totipotent-like fate potential. the miR-34a/Gata2 pathway that represses MERVL expression also restricts the acquisition of totipotency in pluripotent stem cell culture. Taken together, our findings provide novel insights into the molecular basis for totipotent-like cell fate potential, and highlight the functional importance of miRNAs in regulating cell fate plasticity in pluripotent stem cell culture.