[생명공학연구소] 2017년도 제3차 세미나 개최
- 일시 : 2017.05.04
[생명공학연구소] 2017년도 제3차 세미나 개최
생명공학연구소에는 다음과 같이 세미나를 개최하고자 하오니 교수님들과 학생 여러분의 많은 참여 바랍니다.
일시 :: 2017년 5월 4일 오전 12:00 ~ 13:15
장소 :: 생명응용과학대학 제3실험동 201호
연사 :: 최정규 교수(차의과대학 의생명공학)
주제 :: The Crucial Role of Mechanical Heterogeneity in Regulating Follicle Development and Ovulation with Engineered Ovarian Microtissue
초록 ::
Contemporary systems for in vitro culture of ovarian follicles do not recapitulate the mechanical heterogeneity in mammalian ovary. Here we report microfluidic generation of biomimetic ovarian microtissue for miniaturized three-dimensional (3D) culture of early secondary preantral follicles by using alginate (harder) and collagen (softer) to fabricate the ovarian cortical and medullary tissues, respectively. This biomimetic configuration greatly facilitates follicle development to antral stage. Moreover, it enables in vitro ovulation of cumulus-oocyte complex (COC) from the antral follicles in the absence of luteinizing hormone (LH) and epidermal growth factor (EGF) that are well accepted to be responsible for ovulation in contemporary literature. These data reveal the crucial role of mechanical heterogeneity in the mammalian ovary in regulating follicle development and ovulation.
The biomimetic ovarian microtissue and the microfluidic technology developed in this study are valuable for improving in vitro culture of follicles to preserve fertility and for understanding the mechanism of follicle development and ovulation to facilitate the search of cures to infertility due to ovarian disorders.